OX40 Effector Cells (CPM)

The OX40 Bioassay, (Cat.# J2172), is a bioluminescent cell-based assay that can be used to measure the potency and stability of ligands or agonist antibodies that can bind and activate OX40. The assay consists of a genetically engineered Jurkat T cell line that expresses human OX40 and a luciferase reporter driven by a response element that can respond to OX40 ligand/agonist antibody stimulation. The OX40 Effector Cells are provided in Cell Propagation Model (CPM) format, which includes cryopreserved cells that can be thawed, propagated and banked for long-term use. The OX40 Bioassay, Propagation Model, reflects the mechanism of action (MOA) of biologics designed to activate OX40. Specifically, OX40-mediated luminescence is detected following the addition of OX40 ligand and OX40 agonist antibodies. The bioassay is prequalified according to ICH guidelines and shows the precision, accuracy and linearity required for routine use in potency and stability studies.