PC-12 nuclear extract (Forskolin stimulated)
Active Motif's PC-12 nuclear extract (Forskolin stimulated) was prepared from cell cultures of the rat adrenal pheochromocytoma PC-12 cell line. This cell line originated from a catecholamine-secreting tumor (pheochromocytoma) of the rat adrenal medulla. In addition to catecholamine, PC-12 cells also produce neurotransmitters. PC-12 cells are frequently used as a model system for neuronal differentiation because of their ability to differentiate into a sympathetic neuronal phenotype in response to nerve growth factor, fibroblast growth factor and, under certain conditions, epidermal growth factor. Forskolin is a diterpene compound that activates adenylyl cyclase, thus elevating intracellular levels of cAMP. Activation of the cAMP signaling pathway is associated with the regulation of a number of cellular processes, including motility, neurite outgrowth, metabolism, transcription, and neurotransmitter secretion. Similar to nerve growth factor stimulation of the MAPK signaling pathway, forskolin stimulation leads to PKA-dependent phosphorylation and activation of the CREB transcription factor. In the brain, CREB function is primarily associated with long-term potentiation in the formation of memories. Disturbance of CREB function in the brain is linked to the progression of Huntington's Disease and Rubinstein-Taybi syndrome, as well as some forms of cancer.