The pF12K RM Flexi Vector is part of the Flexi Vector System and a component of the Regulated Mammalian Expression System (Promega Cat. 9470). High levels of expression can be achieved upon induction with coumermycin. All Flexi vectors carry the lethal barnase gene, which is replaced by the DNA fragment of interest and acts as a positive selection for successful ligation of the insert. This vector provides kanamycin resistance in E. coli.