pGL4.45[luc2P/ISRE/Hygro] Vector

Creating a cell line with an indicator of a functional signaling pathway is useful for deciphering the components in a signaling pathway. These tools are made by inserting multiple repeats of a response element upstream of a minimal promoter (minP). We offer vectors that report the activity of a variety of cellular stress pathways using the optimized firefly luciferase gene luc2 in the pGL4 backbone. The luc2 gene is engineered to remove most cryptic transcription factor binding sites and improve mammalian expression through codon optimization. The luc2P and luc2CP and RapidResponse genes are luc2 genes appended with degradation sequences to influence the cellular half-life of the luc2 gene. The RapidResponse genes respond more rapidly to stimuli but at the expense of signal intensity. The luc2P (1-hour half-life) gene responds more rapidly than luc2 (3-hour half-life) with moderate signal intensity. These response element vectors also have a hygromycin resistance selectable marker for use either in transient transfection experiments or for selection of a stable cell line. To speed research, several pre-designed response element vectors based on the pGL4.27 Vector are available. Some of these also are available stable cell lines (GloResponse Cell Lines). The cytokine signaling pathway vectors available include interferon-alpha (INF-alpha) in pGL4.45[luc2P/ISRE/Hygro] Vector, interleukin 6 (IL-6) in pGL4.47[luc2P/SIE/Hygro] Vector, transforming growth factor beta (TGF-beta) in pGL4.48[luc2P/SBE/Hygro] Vector and interleukin 3 (IL-3) in pGL4.52[luc2P/STAT5RE/Hygro] Vector.