The PowerPlex 16 System is a multiplex STR system for use in DNA typing, including paternity testing, forensic DNA analysis, human identity testing and tissue culture strain identification. The system allows the co-amplification and three-color detection of sixteen loci (fifteen STR loci and Amelogenin): Penta E, D18S51, D21S11, TH01, D3S1358, FGA, TPOX, D8S1179, vWA, Amelogenin, Penta D, CSF1PO, D16S539, D7S820, D13S317 and D5S818. One primer for each of the Penta E, D18S51, D21S11, TH01 and D3S1358 loci is labeled with fluorescein (FL); one primer for each of the FGA, TPOX, D8S1179, vWA and Amelogenin loci is labeled with carboxy-tetramethylrhodamine (TMR); and one primer for each of the Penta D, CSF1PO, D16S539, D7S820, D13S317 and D5S818 loci is labeled with 6-carboxy-4',5'-dichloro-2',7'-dimethoxy-fluorescein (JOE). All sixteen loci are amplified simultaneously in a single tube and analyzed in a single injection or gel lane. The PowerPlex 16 System is compatible with the ABI PRISM 310, 3100 and 3100-Avant Genetic Analyzers and Applied Biosystems 3130 and 3130xl Genetic Analyzers.