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multi-core-buffer-pack

MULTI-CORE Buffer Pack

by Promega
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  • The MULTI-CORE™ Buffer Pack contains convenient aliquots of the Promega universal restriction enzyme 10X buffer. The MULTI-CORE™ Buffer is formulated to provide simple buffering conditions for performing multiple digestions. Many Promega restriction enzymes have between 50% and 100% activity in reactions using MULTI-CORE™ Buffer.

    See the table of Promega 1X Restriction Enzyme Buffer Composition below.

    1X Restriction Enzyme Buffer Composition.

    Buffer pH (at 37°C) Tris-HCl (mM) MgCl2 (mM) NaCl (mM) KCl (mM) DTT (mM)
    A 7.5 6 6 6 — 1
    B 7.5 6 6 50 — 1
    C 7.9 10 10 50 — 1
    D 7.9 6 6 1,500 — 1
    E 7.5 6 6 100 — 1
    F 8.5 10 10 100 — 1
    G 8.2 50 5 — — —
    H 7.5 90 10 50 — —
    J 7.5 10 7 — 50 1
    K 7.4 10 10 — 150 1
    L 9.0 10 3 100 — —

    MULTI-CORE™ Buffer (1X) = 25mM Tris-acetate (pH 7.5 at 37°C), 100mM potassium acetate, 10mM magnesium acetate, 1mM DTT.

    Notes:

    1. For each 10°C rise in temperature between 0°C and 25°C, the pH of Tris buffers decreases 0.31 pH units.
    2. For each 10°C rise in temperature between 25°C and 37°C, the pH of Tris buffers decreases 0.25 pH units.
    3. All restriction enzymes are supplied with 10mg/ml Acetylated BSA. Although BSA is not absolutely required for activity, it has been shown to enhance activity of many restriction enzymes. We recommend adding BSA to all restriction digests at a final concentration of 0.1mg/ml.

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    Storage Conditions

    -30C TO -10C

    Use Restrictions

    For Research Use Only. Not for Use in Diagnostic Procedures.