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MultiTox-Fluor Multiplex Cytotoxicity Assay

by Promega
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  • Measure the Number of Live and Dead Cells Simultaneously

    The single-reagent-addition, homogeneous, fluorescent assay measures cell viability and cytotoxicity by detecting two distinct protease activities. The live-cell protease activity is restricted to intact viable cells and is measured using a fluorogenic, cell-permeant peptide substrate (GF-AFC Substrate). The substrate enters intact cells where it is cleaved to generate a fluorescent signal proportional to the number of living cells. This live-cell protease activity marker becomes inactive upon loss of membrane integrity and leakage into the surrounding culture medium. A second, cell-impermeant, fluorogenic peptide substrate (bis-AAF-R110 substrate) is used to measure dead-cell protease activity that has been released from cells that have lost membrane integrity. 

    The homogeneous, "add-mix-measure" protocol eliminates parallel plate processing and reduces cell culture costs, and the ratio of live:dead cells is independent of cell number and normalizes data. Data normalization for cell number makes results more comparable well-to-well, plate-to-plate, day-to-day.

    Complementary live- and dead-cell measures with independent chemistries serve as internal controls for each other, and multiplexing capability with most Promega bioluminescent cell-based apoptosis or genetic reporter assays results in more data per well.

    The ratiometric response obtained from the MultiTox-Fluor Assay technology.
    The ratiometric response obtained from the MultiTox-Fluor Assay technology.

    Notes

    Cat # G9200 contains sufficient reagents for 100 assays at 100ul/assay in a 96-well format or 400 assays at 25ul/assay in a 384-well format. Cat # G9201 contains sufficient reagents for 500 assays at 100ul/assay in a 96-well format or 2,000 assays at 25ul/assay in a 384-well format. Cat # G9202 contains sufficient reagents for 1,000 assays at 100ul/assay in a 96-well format or 4,000 assays at 25ul/assay in a 384-well format.

  • Protocols

    Complete Protocol

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    MultiTox-Fluor Multiplex Cytotoxicity Assay

    PDF (1 MB)

  • Certificate of Analysis

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    Storage Conditions

    -30C TO -10C

    Use Restrictions

    For Research Use Only. Not for Use in Diagnostic Procedures.

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