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Mung Bean Nuclease

by Promega
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  • Mung Bean Nuclease catalyzes the degradation of single-stranded DNA and RNA endonucleolytically to yield 5´ phosphoryl-terminated products. While the nuclease prefers ssDNA over dsDNA by 30,000-fold, at very high concentrations the enzyme degrades double-stranded DNA from both ends. Mung Bean Nuclease has been used for transcript mapping studies, for flushing staggered ends and for the separation of cDNA strands after synthesis with reverse transcriptase and DNA Polymerase I. Mung Bean Nuclease is provided with 10X Reaction Buffer: 300mM sodium acetate (pH 5.0 at 15°C), 500mM NaCl, 10mM ZnCl2.

    References

    1. Ardelt, W. and Laskowski, M., Sr. (1971) Biochem. Biophys. Res. Comm. 44, 1205–11.
    2. Kroeker, W.D. et al. (1976) Biochemistry 15, 4463–7.
    3. Mathis, D.J. et al. (1981) Proc. Natl. Acad. Sci. USA 78, 7383–7.
    4. Green, M.R. and Roeder, R.G. (1980) Cell 22, 231–42.
    5. Gubler, U. (1987) Meth. Enzymol. 152, 330–5.

    Storage Buffer: 10mM Tris-HCl (pH 7.5 at 25°C), 50mM NaCl, 0.01% Triton® X-100 and 50% glycerol.

    Source: Mung bean sprouts.

    QC Tests: Activity, endonuclease.

    Unit Definition: One unit is defined as the amount of enzyme required to produce 1ug of acid-soluble nucleotides per minute at 37°C in 30mM sodium acetate (pH 5.0), 50mM NaCl, 1mM ZnCl2, 0.5mg/ml denatured calf thymus DNA and 5% glycerol.

  • Protocols

    Complete Protocol

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    Mung Bean Nuclease Protocol

    PDF (110 KB)

  • Certificate of Analysis

    Lookup Certificate of Analysis

    Storage Conditions

    -30C TO -10C

    Use Restrictions

    For Research Use Only. Not for Use in Diagnostic Procedures.