- Overview
- Protocols
- Specifications
- Resources
NAD/NADH-Glo™ Assay and NADP/NADPH-Glo™ Assays
The NAD/NADH-Glo and NADP/NADPH-Glo Assays are bioluminescent, homogeneous, single-reagent-addition methods to rapidly detect NAD+ and NADH and NADP+ and NADPH levels in cells and enzymatic reactions. Current methods to detect nicotinamide adenine dinucleotides lack sensitivity and reproducibility and require sample manipulation and large numbers of cells for cell-based applications. The assay is easily adapted for inhibitor screening in high-throughput formats. The sensitivity and robustness of the assay chemistry allow fewer cells to be used to detect individual nucleotides directly in multiwell plates or analysis of enzymes with low activity or with low Km values.
Low nanomolar sensitivity allows direct in-well detection and minimizes the amount of cells or enzyme required
Assay NAD/NADH-Glo™ Assay NADP/NADPH-Glo™ Assay Limit of Detection (LOD) 1nM (25fmol/25µl) 1nM (25fmol/25µl) Linearity 1–500nM 1–500nM Signal-to-background ratio (S/B max) ~250 ~250 Cells/well for total dinucleotides 500–25,000 500–12,000 Cells/well for individual dinucleotides* 2,000–100,000 2,000–100,000 *Starting amount of cells; 1/4 of sample can be used to detect individual nucleotides *Starting amount of cells; 1/4 of sample can be used to detect individual nucleotides *Starting amount of cells; 1/4 of sample can be used to detect individual nucleotides 
-
Protocols
Complete Protocol
NAD/NADH-Glo™ Assay Technical Manual
PDF (672 KB)
NADP/NADPH-Glo™ Assay Technical Manual
PDF (642 KB)
-
Certificate of Analysis
Lookup Certificate of AnalysisStorage Conditions
LESS THAN -65C
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.
-
Resources
Articles
- NAD+: It's Not Just Metabolism Anymore
- NAD Detection Assays: Technology and Features
- Bioluminescent Nicotinamide Adenine Dinucleotide Detection Assays Part II: Choosing the Right Product for Your Application
Citations
-
Regulation of cancer stem cell metabolism by secreted frizzled-related protein 4 (sFRP4).
--
2018 Cancers (Basel)
Other
