NanoBRET Positive Control
The NanoBRET™ Positive Control Vector encodes a NanoLuc®-HaloTag® fusion protein that tethers together the NanoLuc® donor and HaloTag® acceptor proteins to ensure efficient energy transfer. The NanoBRET™ Positive Control Vector should be diluted in Transfection Carrier DNA to reduce cellular expression levels due to the high NanoBRET™ signal produced. The control vector can be used when performing instrument setup or as a reference control when performing other NanoBRET™ assays.
The NanoBRET™ Positive Control contains the following key features:
- NanoLuc®-HaloTag® fusion gene.
- Kanamycin resistance gene for selection in bacteria.
- A CMV promoter for high-level expression of NanoLuc®-HaloTag® fusion in mammalian cells.
The Transfection Carrier DNA contains the following key features:
- Ampicillin resistance gene for selection in bacteria.
Certificate of AnalysisLookup Certificate of Analysis
-30C TO -10C
For Research Use Only. Not for Use in Diagnostic Procedures.