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NanoBRET Positive Control

by Promega
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  • Overview
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  • The NanoBRET™ Positive Control Vector encodes a NanoLuc®-HaloTag® fusion protein that tethers together the NanoLuc® donor and HaloTag® acceptor proteins to ensure efficient energy transfer. The NanoBRET™ Positive Control Vector should be diluted in Transfection Carrier DNA to reduce cellular expression levels due to the high NanoBRET™ signal produced. The control vector can be used when performing instrument setup or as a reference control when performing other NanoBRET™ assays.

    The NanoBRET™ Positive Control contains the following key features:

    • NanoLuc®-HaloTag® fusion gene.
    • Kanamycin resistance gene for selection in bacteria.
    • A CMV promoter for high-level expression of NanoLuc®-HaloTag® fusion in mammalian cells.

    The Transfection Carrier DNA contains the following key features:

    • Ampicillin resistance gene for selection in bacteria.

  • Certificate of Analysis

    Lookup Certificate of Analysis

    Storage Conditions

    -30C TO -10C

    Use Restrictions

    For Research Use Only. Not for Use in Diagnostic Procedures.

  • Resources

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