- Overview
- Protocols
- Specifications
- Resources
NanoBRET™ Technology for Target Engagement
The NanoBRET™ Target Engagement (TE) Assay measures compound binding at select target proteins in intact cells, in real time. The NanoBRET™ TE Assay uses four key components: An expressed cellular target protein that is fused to the bright NanoLuc® luciferase; a cell-permeable fluorescent tracer that specifically binds to the target protein; a substrate for NanoLuc® luciferase; and a cell-impermeable inhibitor for NanoLuc® luciferase.
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Protocols
Complete Protocol
NanoBRET™ Target Engagement Intracellular BET BRD Assay Technical Manual
PDF (2 MB)
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Certificate of Analysis
Lookup Certificate of AnalysisStorage Conditions
LESS THAN -65C
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.
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Resources
Citations
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A luminescent assay for real-time measurements of receptor endocytosis in living cells.
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2015 Anal. Biochem.
Other
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