nanobret-te-intracellular-bet-brd-complete-kit

NanoBRET TE Intracellular BET BRD Complete Kit

by Promega

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Overview
Protocols
Specifications
Resources

NanoBRET™ Technology for Target Engagement

The NanoBRET™ Target Engagement (TE) Assay measures compound binding at select target proteins in intact cells, in real time. The NanoBRET™ TE Assay uses four key components: An expressed cellular target protein that is fused to the bright NanoLuc® luciferase; a cell-permeable fluorescent tracer that specifically binds to the target protein; a substrate for NanoLuc® luciferase; and a cell-impermeable inhibitor for NanoLuc® luciferase.
Protocols

Complete Protocol

NanoBRET™ Target Engagement Intracellular BET BRD Assay Technical Manual

PDF (2 MB)
Certificate of Analysis
Lookup Certificate of Analysis
Storage Conditions

LESS THAN -65C

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.
Resources

Citations
- A luminescent assay for real-time measurements of receptor endocytosis in living cells.
  
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  2015 Anal. Biochem.
Other
- Live-Cell Target Engagement and Residence Time
- NanoBRET™ Target Engagement Vectors