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- Overview
- Protocols
- Specifications
- Resources
State-of-the-Art Reporter Technology brings Greatest Sensitivity and Flexibility
The Nano-Glo® Dual-Luciferase® Reporter (NanoDLR) Assay allows you to detect the activities of firefly and NanoLuc® luciferases in a single sample. Improved firefly chemistry and the bright, sensitive NanoLuc® luciferase make this a highly sensitive dual assay that is simple to use, provides superior data quality, and allows greater flexibility in assay design.
In the NanoDLR Assay protocol, firefly luciferase activity is measured first using the ONE-Glo™ EX Luciferase Assay Reagent. NanoDLR™ Stop & Glo® Reagent is then added to quench the firefly signal and provide the substrate needed to measure NanoLuc® luciferase activity.
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Protocols
Complete Protocol
Nano-Glo® Dual-Luciferase® Reporter Assay Technical Manual
PDF (1 MB)
Quick Protocols
NanoGlo Dual Luciferase Reporter NanoDLR Assay Quick Protocol
PDF (259 KB)
Video Protocols
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Certificate of Analysis
Lookup Certificate of AnalysisStorage Conditions
COMPONENTS AT DIFFERENT TEMPS
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.
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Resources
Articles
- Multiplexing with NanoDLR and CellTiter-Fluor Assays
- Designing a Bioluminescent Reporter Assay: Normalization
- Detection of HIF1? Expression at Physiological Levels Using NanoLuc® Luciferase and the GloMax® Discover System
Citations
