- Overview
- Protocols
- Specifications
- Resources
Simple, Sensitive Bioluminescent Protein Quantitation
The Nano-Glo® HiBiT Lytic Detection System quantifies cellular protein in minutes with high sensitivity and a broad dynamic range using a simple add-mix-read assay format.
The 11 amino acid HiBiT peptide tag can be added easily to a protein of interest, and the total amount of HiBiT-tagged protein in cells measured by adding the Nano-Glo® HiBiT Lytic Detection Reagent.
The detection reagent contains the complementing polypeptide LgBiT, which spontaneously interacts with the HiBiT tag to reconstitute the bright, luminescent NanoBiT® enzyme. The luminescence intensity is directly proportional to the amount of HiBiT-tagged protein in the cell lysate over seven orders of magnitude. The glow-type luminescent signal is stable for hours.
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Protocols
Complete Protocol
Nano-Glo® HiBiT Lytic Detection System Technical Manual
PDF (1 MB)
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Certificate of Analysis
Lookup Certificate of AnalysisStorage Conditions
-30C TO -10C
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.
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Resources
Articles
- Protocol for Adding HiBiT Using CRISPR
- Monitoring HIF1A stabilization and BRD4 degradation using HIBiT protein tags.
Citations
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Development of a rapid and quantitative method for the analysis of viral entry and release using the NanoLuc® luciferase complementation assay.
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2017 Virus Res.
Posters
