Nano-Glo Live Cell Assay System
Single-Addition Reagent to Detect NanoLuc® Luminescence in Live Cells
The Nano-Glo® Live Cell Assay System is a single-addition, non-lytic detection reagent used to measure NanoBiT® or NanoLuc® luminescence from living cells. The reagent is prepared by diluting the Nano-Glo® Live Cell Substrate with the Nano-Glo® LCS Dilution Buffer to make the Nano-Glo® Live Cell Reagent, a 5X stock that is added directly to cell culture medium. Both substrate and buffer solutions are optimized to provide enhanced stability. The Nano-Glo® Live Cell Reagent is designed to reduce autoluminescence in the presence or absence of serum, increasing the sensitivity for detection of low levels of NanoBiT® or NanoLuc® luminescence. The Nano-Glo® Live Cell Assay System can be used to monitor luminescence at a user-defined time point or continuously for up to 2 hours without compromising cell viability.
Cat.# N2011 is sufficient for 100 assays in 96-well plates; Cat.# N2012 is sufficient for 1,000 assays in 96-well plates, Cat.# N2013 is sufficient for 10,000 assays in 96-well plates.
The signal stability observed after addition of the Nano-Glo® Live Cell Reagent to culture media can be influenced by experimental parameters such as high levels of enzyme activity, temperature or the presence of FBS in the media. Please refer to the Technical Manual for additional information.
FB195 Nano-Glo Live Cell Assay System
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Certificate of AnalysisLookup Certificate of Analysis
-30C TO -10C
For Research Use Only. Not for Use in Diagnostic Procedures.