Skip to content

Nano-Glo Live Cell Assay System

by Promega
Login to view price.
Size
  • Overview
  • Protocols
  • Specifications
  • Resources
  • Single-Addition Reagent to Detect NanoLuc® Luminescence in Live Cells

    The Nano-Glo® Live Cell Assay System is a single-addition, non-lytic detection reagent used to measure NanoBiT® or NanoLuc® luminescence from living cells. The reagent is prepared by diluting the Nano-Glo® Live Cell Substrate with the Nano-Glo® LCS Dilution Buffer to make the Nano-Glo® Live Cell Reagent, a 5X stock that is added directly to cell culture medium. Both substrate and buffer solutions are optimized to provide enhanced stability. The Nano-Glo® Live Cell Reagent is designed to reduce autoluminescence in the presence or absence of serum, increasing the sensitivity for detection of low levels of NanoBiT® or NanoLuc® luminescence. The Nano-Glo® Live Cell Assay System can be used to monitor luminescence at a user-defined time point or continuously for up to 2 hours without compromising cell viability.

    Cat.# N2011 is sufficient for 100 assays in 96-well plates; Cat.# N2012 is sufficient for 1,000 assays in 96-well plates, Cat.# N2013 is sufficient for 10,000 assays in 96-well plates.

    The signal stability observed after addition of the Nano-Glo® Live Cell Reagent to culture media can be influenced by experimental parameters such as high levels of enzyme activity, temperature or the presence of FBS in the media. Please refer to the Technical Manual for additional information.

    Signal stability of the Nano-Glo® Live Cell Reagent in HEK293 cells expressing varying levels of NanoLuc® Luciferase.
    Signal stability of the Nano-Glo® Live Cell Reagent in HEK293 cells expressing varying levels of NanoLuc® Luciferase.
  • Protocols

    Quick Protocols

    Download PDF

    FB195 Nano-Glo Live Cell Assay System

    PDF (482 KB)

  • Certificate of Analysis

    Lookup Certificate of Analysis

    Storage Conditions

    -30C TO -10C

    Use Restrictions

    For Research Use Only. Not for Use in Diagnostic Procedures.

  • Resources

    Citations

    Posters