NarI

Cut Site:

GG^?CG   CC
CC   GC_?GG

Usage Notes:

1. NarI exhibits site preferences and may not cut to completion.
2. Ends generated by NarI can be directly ligated to ends generated by Hsp92I, ClaI or HpaII. The NarI site may not be regenerated in the ligation product.

Incubation Conditions: Buffer G. 37°C.

Source: Nocardia argentinensis.

Storage Buffer: 10mM Tris-HCl (pH 7.4), 300mM NaCl, 0.1mM EDTA, 1mM DTT, 0.5mg/ml BSA, 50% glycerol.

Percent Activity in 4-CORE® Buffer:

Frequency of Cutting: