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NciI

by Promega
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  • Overview
  • Protocols
  • Specifications
  • Resources
  • Cut Site:

    CC?(C/G)  GG
    GG  (G/C)?CC

    Notes:

    1. Star activity may be observed with salt conditions <50mm.
    2. NciI produces a single-base overhang that is difficult to ligate.

    Incubation Conditions: Buffer B. 37°C.

    Source: Neisseria cinerea ATCC 14685.

    Storage Buffer: 10mM Tris-HCl (pH 7.4), 50mM NaCl, 0.1mM EDTA, 1mM DTT, 0.5mg/ml BSA, 50% glycerol.

    Percent Activity in 4-CORE® Buffer:

    A B C D MULTI-CORE™
    100%* 100% 25–50% 25–50% 50–75%

    *Buffer A is not recommended due to potential star activity.

    Frequency of Cutting:

    ? Ad-2 ?X174 pUC18 M13mp18 pBR322
    114 97 1 7 4 10
  • Protocols

    Complete Protocol

    Download PDF

    Restriction Enzyme Protocol

    PDF (410 KB)

  • Certificate of Analysis

    Lookup Certificate of Analysis

    Storage Conditions

    -30C TO -10C

    Use Restrictions

    For Research Use Only. Not for Use in Diagnostic Procedures.

  • Resources

    Other