- Overview
- Protocols
- Specifications
- Resources
The electrophoretic mobility shift assay (EMSA, gel shift, gel retardation) is a relatively simple and sensitive method to investigate protein:DNA interactions. These oligonucleotides contain consensus DNA-binding sites for individual sequence-specific transcription factors. The double-stranded oligonucleotides are designed with 5´ OH blunt ends, making them easily labeled to high specific activity with T4 polynucleotide kinase.
Applications include gel-shift assays and affinity chromatography.-
Protocols
Complete Protocol
Gel Shift Assay System Technical Bulletin
PDF (199 KB)
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Certificate of Analysis
Lookup Certificate of AnalysisStorage Conditions
-30C TO -10C
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.
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Resources
Citations