nf-kappab-consensus-oligonucleotide

NF-kappaB Consensus Oligonucleotide

by Promega

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Overview
Protocols
Specifications
Resources

The electrophoretic mobility shift assay (EMSA, gel shift, gel retardation) is a relatively simple and sensitive method to investigate protein:DNA interactions. These oligonucleotides contain consensus DNA-binding sites for individual sequence-specific transcription factors. The double-stranded oligonucleotides are designed with 5´ OH blunt ends, making them easily labeled to high specific activity with T4 polynucleotide kinase.
Applications include gel-shift assays and affinity chromatography.
Protocols

Complete Protocol

Gel Shift Assay System Technical Bulletin

PDF (199 KB)
Certificate of Analysis
Lookup Certificate of Analysis
Storage Conditions

-30C TO -10C

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.
Resources

Citations
- Mitogenic responses of vascular smooth muscle cells to lipid peroxidation-derived aldehyde 4-hydroxy-trans-2-nonenal (HNE): Role of aldose reductase-catalyzed reduction of the HNE-glutathione conjugates in regulating cell growth.
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  2006 J. Biol. Chem.