NheI

Cut Site:

G^?CTAG   C
C   GATC_?G

Notes:

1. For site-specific methylation information, consult McClelland, M., Nelson, M. and Raschke, E. (1994) Nucl. Acids Res. 22, 3640–59.
2. Ends generated with NheI can be directly ligated to ends generated by SpeI or XbaI. None of these recognition sites are regenerated in the ligation product.

Incubation Conditions: Buffer B. 37°C.

Source: Neisseria mucosa heidelbergensis.

Storage Buffer: 10mM Tris-HCl (pH 7.4), 50mM NaCl, 0.1mM EDTA, 1mM DTT, 0.5mg/ml BSA, 50% glycerol.

Percent Activity in 4-CORE® Buffer:

Frequency of Cutting: