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NotI

by Promega
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Size
  • Overview
  • Protocols
  • Specifications
  • Resources
  • Cut Site:

    GC?GGCC  GC
    CG  CCGG?CG

    Choose Your Configuration: Learn more about our custom options for this product at: www.promega.com/custom/

    Usage Note: Ends generated using NotI can be directly ligated to ends generated using BstZI or Eco52I. BstZI and Eco52I sites are regenerated in the ligation product. NotI sites may not be regenerated.

    Incubation Conditions: Buffer D. 37°C.

    Source: Nocardia otitidis-caviarum.

    Storage Buffer: 10mM Tris-HCl (pH 7.4), 0.1% Triton® X-100, 500mM KCl, 0.1mM EDTA, 1mM DTT, 0.5mg/ml BSA, 50% glycerol.

    Percent Activity in 4-CORE® Buffer:

    A B C D MULTI-CORE™
    <10% 10–25% 25–50% 100% 25–50%

    Frequency of Cutting:

    ? Ad-2 ?X174 pUC18 M13mp18 pBR322
    0 7 0 0 0 0
  • Protocols

    Complete Protocol

    Download PDF

    Restriction Enzyme Protocol

    PDF (410 KB)

  • Certificate of Analysis

    Lookup Certificate of Analysis

    Storage Conditions

    -30C TO -10C

    Use Restrictions

    For Research Use Only. Not for Use in Diagnostic Procedures.

  • Resources

    Other