- Overview
- Protocols
- Specifications
- Resources
Cut Site:
GC?GGCC GC
CG CCGG?CGChoose Your Configuration: Learn more about our custom options for this product at: www.promega.com/custom/
Usage Note: Ends generated using NotI can be directly ligated to ends generated using BstZI or Eco52I. BstZI and Eco52I sites are regenerated in the ligation product. NotI sites may not be regenerated.
Incubation Conditions: Buffer D. 37°C.
Source: Nocardia otitidis-caviarum.
Storage Buffer: 10mM Tris-HCl (pH 7.4), 0.1% Triton® X-100, 500mM KCl, 0.1mM EDTA, 1mM DTT, 0.5mg/ml BSA, 50% glycerol.
Percent Activity in 4-CORE® Buffer:
A B C D MULTI-CORE™ <10% 10–25% 25–50% 100% 25–50% Frequency of Cutting:
? Ad-2 ?X174 pUC18 M13mp18 pBR322 0 7 0 0 0 0 -
Protocols
Complete Protocol
Restriction Enzyme Protocol
PDF (410 KB)
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Certificate of Analysis
Lookup Certificate of AnalysisStorage Conditions
-30C TO -10C
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.
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Resources
Other