- Overview
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- Specifications
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Cut Site:
TCG?CGA
AGC?GCTNotes:
- Sensitive to overlapping dam methylation.
- NruI replaces SpoI.
Incubation Conditions: Buffer K. 37°C.
Source: Nocardia rubra ATCC 15906.
Storage Buffer: 10mM Tris-HCl (pH 7.4), 50mM KCl, 0.1mM EDTA, 1mM DTT, 0.5mg/ml BSA, 50% glycerol.
Percent Activity in 4-CORE® Buffer:
A B C D K MULTI-CORE™ <10% <10% <10% 50–75% 100% 10–25% Frequency of Cutting:
? Ad-2 ?X174 pUC18 M13mp18 pBR322 5 5 2 0 0 1 -
Protocols
Complete Protocol
Restriction Enzyme Protocol
PDF (410 KB)
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Certificate of Analysis
Lookup Certificate of AnalysisStorage Conditions
-30C TO -10C
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.
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Resources
Other