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ONE-Glo Luciferase Assay System

by Promega
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  • Overview
  • Protocols
  • Specifications
  • Resources
  • Bright, Stable Luminescence Signal for Batch and Continuous Processing Systems

    The ONE-Glo™ Luciferase Assay System provides a highly sensitive, robust, homogeneous assay for detection of firefly luciferase reporter gene expression in mammalian cells. Ideally suited for high- and ultrahigh-throughput applications, the ONE-Glo™ Assay contains a new luciferase substrate, resulting in a reagent that is more stable, more tolerant to sample components, and has less odor than standard luciferase assay reagents. These features ensure that the ONE-Glo™ Assay provides robust performance and also eliminates many of the handling inconveniences experienced using other reporter assays in a high-throughput setting.

    Assay Advantages

    • Room temperature or 4°C storage options. The extended stability of the ONE-Glo™ Reagent makes it more convenient for everyday use.
    • Improved Assay Precision. The ONE-Glo™ Reagent is less sensitive to mixing and dispensing conditions, enhancing reproducibility. Ideal for use in high-density (384- and 1536-well) microplates.
    • Brighter, Longer-Lasting Signal. Optimized for batch and continuous-process handling, the extended bright light output allows high sensitivity, especially for extended incubations.
    • Reduced Unwanted Effects from Sample Components. The ONE-Glo™ Assay is less sensitive to culture media, phenol red and luciferase inhibitors than other luciferase assays.
    Extended bright light output allows high-sensitivity assays to be run in batch or continuous operations.

    Extended Stability at 22°C

    Extended Stability at 22°C
    Reconstituted ONE-Glo™ Reagent can be used much longer than other bright-type reagents.

    Phenol Red Stability

    Phenol Red Stability
    ONE-Glo™ Reagent is more tolerant of phenol red than other luciferin-based reagents.
  • Protocols

    Complete Protocol

    Download PDF

    ONE-Glo™ Luciferase Assay System Technical Manual

    PDF (364 KB)

    Video Protocols

  • Certificate of Analysis

    Lookup Certificate of Analysis

    Storage Conditions

    -30C TO -10C

    Use Restrictions

    For Research Use Only. Not for Use in Diagnostic Procedures.

  • Resources