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pCAT3-Control Vector

by Promega
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  • Overview
  • Protocols
  • Specifications
  • Resources
  • The pCAT™3 Reporter Vectors provide a basis for the quantitative analysis of factors that may regulate mammalian gene expression. The redesigned backbone of the pCAT™3 Reporter Vectors is similar to the pGL3 Luciferase Vectors with the exception of a chimeric intron located 5´ of the chloramphenicol acetyltransferase (CAT) gene. As with the pGL3 Vectors, the pCAT™3 Vectors contain a different polyadenylation site located 3´ of the gene. The redesigned backbone increases expression of the reporter gene, improves in vivo vector stability and provides greater flexibility in performing manipulations. The vectors offer optimal translation efficiency, and the multiple cloning sites are compatible with the pGL3 Luciferase Reporter Vectors

    About the Vector Options

    The reporter vectors are available in four options, depending on your needs. The Basic Vector has the reporter gene and restriction enzymes sites to clone in a promoter of interest. The Enhancer Vector contains the SV40 Enhancer Region for putative promoters that may need an additional regulator region. The Promoter Vector is suitable for cloning and testing putative enhancer regions. The Control Vector contains the SV40 Promoter and Enhancer Region and can be used as a normalization control for expression experiments.

    1131VA08_5A-W

    pCAT™3-Basic Vector GenBank® Accession Number U57024 and vector sequence text file.

    1130VA08_5A-W

    pCAT™3-Control Vector GenBank® Accession Number U57025 and vector sequence text file.

    1129VA08_5A-W

    pCAT™3-Enhancer Vector GenBank® Accession Number U57026  and vector sequence text file.

    1132VA08_5A-W

    pCAT™3-Promoter Vector GenBank® Accession Number U57027 and vector sequence text file.

  • Protocols

    Complete Protocol

    Download PDF

    pCAT®3 Reporter Vectors Technical Manual

    PDF (474 KB)

  • Certificate of Analysis

    Lookup Certificate of Analysis

    Storage Conditions

    -30C TO -10C

    Use Restrictions

    For Research Use Only. Not for Use in Diagnostic Procedures.

  • Resources

    Citations