pCI-neo Mammalian Expression Vector

The pCI-neo Mammalian Expression Vector carries the human cytomegalovirus (CMV) immediate-early enhancer/promoter region to promote strong, constitutive expression of cloned DNA inserts in mammalian cells. A ?-globin/IgG chimeric intron located downstream from the enhancer/promoter region can further increase expression. The vector is maintained as an episome in cells expressing the SV40 large T antigen, leading to even higher levels of expression.

This vector also contains the neomycin phosphotransferase gene, a selectable marker for stable transfection of mammalian cells. The pCI-neo Vector can be used for transient or stable expression by selecting transfected cells with the antibiotic G-418. Multiple cloning sites exist for easy insertion of cDNA.

References

1. Brinster, R.L. et al. (1988) Proc. Natl. Acad. Sci. USA 85, 836–40.
2. Choi, T. et al. (1991) Mol. Cell. Biol. 11, 3070–4.
3. Palmiter, R.D. et al. (1991) Proc. Natl. Acad. Sci. USA 88, 478–82.
4. Carswell, S. and Alwine, J.C. (1989) Mol. Cell. Biol. 9, 4248–58.

pCI-neo Mammalian Expression Vector GenBank® Accession Number U47120 and vector sequence text file.