- Overview
- Protocols
- Specifications
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Express Cloned Genes In Vitro or In Vivo
The pCMVTnT™ and pTnT™ Vectors are designed for convenient expression of cloned genes in vitro or in vivo. SP6 and T7 promoters allow expression from SP6- or T7-based coupled in vitro transcription/translation systems. The presence of RNA phage promoters also allows highly efficient synthesis of RNA in vitro. Both vectors contain a 5´ ?-globin leader sequence and synthetic poly(A)30 tail, which have been shown to enhance expression of certain genes. The multiple cloning site provides a selection of restriction sites.
For in vivo expression, the pCMVTnT™ Vector contains a CMV enhancer/promoter region, which allows strong constitutive expression in many cell types.
pTnT™ Vector GenBank® Accession Number AF479322 and vector sequence text file. pCMVTnT™ Vector GenBank® Accession Number AF477200 and vector sequence text file
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Protocols
Complete Protocol
pCMVTNT™ Vector
PDF (604 KB)
pTNT™ Vector Technical Bulletin
PDF (590 KB)
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Certificate of Analysis
Lookup Certificate of AnalysisStorage Conditions
BELOW -10C
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.
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Resources
Citations