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PCR Nucleotide Mix, 25mM

by Promega
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  • Overview
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  • High-quality deoxynucleotide triphosphates (dNTPs) are critical for PCR efficacy. The PCR Nucleotide Mix is a premixed solution containing the sodium salts of dATP, dCTP, dGTP and dTTP. PCR Nucleotide Mix is manufactured under cGMP conditions and has equimolar amounts of each dNTP to ensure optimal PCR. Adding dNTPs as a mix also simplifies pipetting steps and reduces the risk of contamination.

    There are two ready-to-use formulations available:

    • A premixed solution with each nucleotide at a concentration of 10mM in water at pH 7.5; the total concentration of nucleotides is 40mM.
    • A premixed solution with each nucleotide at a concentration of 25mM in water at pH 7.5; the total concentration of nucleotides is 100mM.

    Promega PCR systems, enzymes and reagents are proven in PCR to ensure reliable, high-performance results. If you are not completely satisfied with any Promega PCR product, we will send a replacement or refund your account.

    Learn more about custom options for this product at: www.promega.com/custom/

    pcr-guaranteegray-circlePerformance Guarantee: Promega PCR systems, enzymes and reagents are proven in PCR to ensure reliable, high-performance results. If you are not completely satisfied with any Promega PCR product, we will send a replacement or refund your account.

  • Protocols

    Complete Protocol

    Download PDF

    PCR Nucleotide Mix Protocol

    PDF (129 KB)

    Download PDF

    PCR Nucleotide Mix, 25mM Protocol

    PDF (130 KB)

  • Certificate of Analysis

    Lookup Certificate of Analysis

    Storage Conditions

    -30C TO -10C

    Use Restrictions

    For Research Use Only. Not for Use in Diagnostic Procedures.