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PDE-Glo Phosphodiesterase Assay

by Promega
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  • Overview
  • Protocols
  • Specifications
  • Resources

    • Versatile:
      • Works with both cAMP and cGMP PDEs.
    • Sensitive:
      • Excellent signal:background ratios.
      • Scalable to 1536-well plate formats.
    • Fast and Easy to Use:
      • Assay can be completed in <1 hour.
      • Homogeneous.
    • Proven Luminescent Technology:
      • Powered by Ultra-Glo™ Luciferase.
      • Non-radioactive.
    • No Interference by Fluorescent Compounds.

    • High-throughput analysis of library compounds to identify inhibitors of either cAMP- or cGMP-specific PDEs.

    The PDE-Glo™ Phosphodiesterase Assay is a luminescent, high-throughput screening (HTS) method for measuring cyclic nucleotide phosphodiesterase (PDE) activity from purified sources. 

    Cyclic nucleotide phosphodiesterases are involved in many cellular processes due to their ability to hydrolyze, and thus control, the levels of the second-messenger signaling molecules cAMP and cGMP.

    The availability of selective inhibitors for PDEs has facilitated their use as tools to study cyclic nucleotide signaling and paved the way to investigate the role of PDEs in cellular and tissue pathologies. The PDE-Glo™ Assay is used for screening library compounds to identify inhibitors of either cAMP- or cGMP-specific PDEs. The assay is designed for 384-well plates, but volumes can easily be scaled for 96- or 1536-well plates. The PDE-Glo™ Phosphodiesterase Assay is optimized to work with both cAMP- and cGMP-dependent phosphodiesterases. The total time required for the assay from start to finish is less than 1 hour after the PDE reaction is complete.

    Assay Advantages

    • Test any PDE: Works with both cAMP and cGMP PDEs.
    • Detect activity easily: No interference from fluorescent compounds.
    • Measure activity directly: Functional test for enzyme activity performed with a low-cost luminometer.
    • Excellent signal:background.
    • Scalable to 1536-well formats.
    Fast and Easy to Use
    • Homogenous assay is complete in <1 hour.
    Proven Luminescence Technology
    • Powered by Ultra-Glo™ Recombinant Luciferase.
    • No interference by fluorescent compounds.
    • Non-radioactive.
  • Protocols

    Complete Protocol

    Download PDF

    PDE-Glo™ Phosphodiesterase Assay Technical Bulletin

    PDF (501 KB)

  • Certificate of Analysis

    Lookup Certificate of Analysis

    Storage Conditions

    -30C TO -10C

    Use Restrictions

    For Research Use Only. Not for Use in Diagnostic Procedures.