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PepTag Non-Radioactive cAMP-Dependent Protein Kinase Assay

by Promega
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  • Overview
  • Protocols
  • Specifications
  • Resources
  • Rapid, Sensitive Detection of Protein Kinase A or Protein Kinase C

    The PepTag® Non-Radioactive Protein Kinase Assay Systems provide use brightly colored fluorescent peptide substrates that are highly specific for PKC (PepTag® C1 Peptide-PLSRTLSVAAK) and PKA (PepTag® A1 Peptide-LRRASLG). Phosphorylation of the peptide alters the net charge from +1 to –1, allowing phosphorylated and nonphosphorylated versions of the substrate to be rapidly separated on an agarose gel at neutral pH. Using fluorescent detection, less than 2ng of purified kinase can be detected in less than 2 hours.

    Use with Purified or Partially Purified Samples

    The PepTag® Systems can detect kinase activity in partially purified samples as well as purified preparations of enzymes, making it a good choice for rapid screening of column fractions or for screening kinase activators and inhibitors. In addition to the assay components, each system includes purified kinase for use as a positive control.

    Low Background

    Because the phosphorylation of the colored peptide supplied with the system is used to measure kinase activity, phosphorylation of other substrates occurring naturally in the sample does not add to the kinase activity measured.
  • Protocols

    Complete Protocol

    Download PDF

    PepTag® Assay Technical Bulletin

    PDF (503 KB)

  • Certificate of Analysis

    Lookup Certificate of Analysis

    Storage Conditions

    LESS THAN -65C

    Use Restrictions

    For Research Use Only. Not for Use in Diagnostic Procedures.

  • Resources

    Citations