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pF1K T7 Flexi Vector

by Promega
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  • Overview
  • Protocols
  • Specifications
  • Resources
  • F1A and pF1K T7 Flexi® Vectors are designed for untagged protein expression in E. coli and cell-free systems using the T7 RNA polymerase promoter. Expression levels depend highly on the nature of the protein.

    About the Flexi® Vector System

    The Flexi® Vector System is a simple, yet powerful, directional cloning method for protein-coding sequences. It is based on two rare-cutting restriction enzymes, SgfI and PmeI, and provides a rapid, efficient and high-fidelity way to transfer protein-coding regions between a variety of Flexi® Vectors without the need to resequence. Directly use recombinant clones and minimize time wasted screening background colonies. The versatile cloning of the Flexi® system means you can choose from a variety of expression systems and fusion tag orientations and then transfer to others as required. Direct transfers can only occur between two N-terminal tagged vectors or from an N-terminal to a C-terminal vector.

    Note: Flexi® Vectors contain the lethal barnase gene to reduce background colonies without inserts during the subcloning procedure. Using the Flexi® Vector Cloning System replaces the barnase gene with your insert. These vectors, as purchased, cannot be cultured in normal laboratory strains of E. coli without an insert.

    Sequence Information

    pF1A T7 Flexi® Vector GenBank® Accession Number AY753576 and vector sequence text file.

    pF1K T7 Flexi® Vector GenBank® Accession Number AY753577 and vector sequence text file.

  • Protocols

    Complete Protocol

    Download PDF

    pF1A T7 Flexi® Vector Product Information

    PDF (131 KB)

    Download PDF

    pF1K T7 Flexi® Vector Product Information

    PDF (174 KB)

    Video Protocols

  • Certificate of Analysis

    Lookup Certificate of Analysis

    Storage Conditions

    -30C TO -10C

    Use Restrictions

    For Research Use Only. Not for Use in Diagnostic Procedures.

  • Resources


    Software and Firmware