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pF3K WG (BYDV) Flexi Vector

by Promega
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  • Overview
  • Protocols
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  • The pF3A and pF3K WG (BYDV) Flexi® Vectors were designed for use with Wheat Germ extracts and contain sequences from the barley yellow dwarf virus (BYDV), an RNA plant virus, upstream and downstream of the protein coding region of interest. The BYDV elements interact with each other, form a closed loop and act synergistically to stimulate translation in wheat germ extracts, bypassing mRNA cap and polyadenylation dependencies. These vectors need no licensing fees or complicated transfer restrictions to use. Just clone your ORFs and conveniently transfer the inserts to a variety of vectors designed for multiple applications.

    About the Flexi® Vector System

    The Flexi® Vector System is a simple, yet powerful, directional cloning method for protein-coding sequences. It is based on two rare-cutting restriction enzymes, SgfI and PmeI, and provides a rapid, efficient and high-fidelity way to transfer protein-coding regions between a variety of Flexi® Vectors without the need to resequence. These in vitro translation Flexi® vectors are designed with special sequences for maximal cell-free protein expression in a specific extract system.

    Note: Flexi® Vectors contain the lethal barnase gene to reduce background colonies without inserts during the subcloning procedure. Using the Flexi® Vector Cloning System replaces the barnase gene with your insert. These vectors, as purchased, cannot be cultured in normal laboratory strains of E. coli without an insert.

    Ready-to-Use HaloTag® Flexi® Vector Clones

    For access to 20,000 human and 15,000 mouse ORF clones with HaloTag® Technology in multiple mammalian expression vectors and custom cloning options, visit: www.kasuza.or.jp.kop/dsearch-e

    Sequence Information

    pF3A WG (BYDV) Flexi® Vector GenBank® Accession Number AY949043 and vector sequence text file.

    pF3K WG (BYDV) Flexi® Vector GenBank® Accession Number AY949044 and vector sequence text file.

  • Protocols

    Complete Protocol

    Download PDF

    pF3A WG (BYDV) Flexi® Vector

    PDF (144 KB)

    Download PDF

    pF3K WG (BYDV) Flexi® Vector

    PDF (137 KB)

    Video Protocols

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  • Certificate of Analysis

    Lookup Certificate of Analysis

    Storage Conditions

    -30C TO -10C

    Use Restrictions

    For Research Use Only. Not for Use in Diagnostic Procedures.

  • Resources

    Citations

    Software and Firmware