pFC14A HaloTag CMV Flexi Vector
Constitutive Expression in Mammalian Cells
The pFC14A and pFC14K HaloTag® CMV Flexi® Vector are configured to append HaloTag® to the carboxyterminus of the protein fusion partner and provide constitutive protein expression in mammalian cells using the human cytomegalovirus (CMV) immediate early enhancer/promoter. The vector can be used for both stable and transient gene expression; for stable expression, cotransfection with a vector containing a selectable marker is required.
Note: Flexi® Vectors contain the lethal barnase gene to reduce background colonies without inserts during the subcloning procedure. Using the Flexi® Vector Cloning System replaces the barnase gene with your insert. These vectors, as purchased, cannot be cultured in normal laboratory strains of E. coli without an insert.Applications
- Clone ORF and conveniently transfer these inserts to a variety of vectors designed for multiple applications.
GenBank® Accession Numbers and Sequence Information
pFC14A HaloTag® CMV GenBank® Accession Number EU113046; pFC14A HaloTag® CMV Vector sequence text file.
pFC14K HaloTag® CMV GenBank® Accession Number EU113047; pFC14K HaloTag® CMV Vector sequence text file.
pFC14A HaloTag® CMV Flexi® Vector Protocol
PDF (194 KB)
pFC14K HaloTag® CMV Flexi® Vector Protocol
PDF (192 KB)
Certificate of AnalysisLookup Certificate of Analysis
-30C TO -10C
For Research Use Only. Not for Use in Diagnostic Procedures.
Software and Firmware