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pFC14A HaloTag CMV Flexi Vector

by Promega
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  • Overview
  • Protocols
  • Specifications
  • Resources
  • Constitutive Expression in Mammalian Cells

    The pFC14A and pFC14K HaloTag® CMV Flexi® Vector are configured to append HaloTag® to the carboxyterminus of the protein fusion partner and provide constitutive protein expression in mammalian cells using the human cytomegalovirus (CMV) immediate early enhancer/promoter. The vector can be used for both stable and transient gene expression; for stable expression, cotransfection with a vector containing a selectable marker is required.

    Note: Flexi® Vectors contain the lethal barnase gene to reduce background colonies without inserts during the subcloning procedure. Using the Flexi® Vector Cloning System replaces the barnase gene with your insert. These vectors, as purchased, cannot be cultured in normal laboratory strains of E. coli without an insert.

    Applications
    • Clone ORF and conveniently transfer these inserts to a variety of vectors designed for multiple applications.

    GenBank® Accession Numbers and Sequence Information 

    pFC14A HaloTag® CMV GenBank® Accession Number EU113046; pFC14A HaloTag® CMV Vector sequence text file.
    pFC14K HaloTag® CMV GenBank® Accession Number EU113047; pFC14K HaloTag® CMV Vector sequence text file.

  • Protocols

    Complete Protocol

    Download PDF

    pFC14A HaloTag® CMV Flexi® Vector Protocol

    PDF (194 KB)

    Download PDF

    pFC14K HaloTag® CMV Flexi® Vector Protocol

    PDF (192 KB)

    Video Protocols

    28440899-HALOTAG-ANIMATION-fallback
  • Certificate of Analysis

    Lookup Certificate of Analysis

    Storage Conditions

    -30C TO -10C

    Use Restrictions

    For Research Use Only. Not for Use in Diagnostic Procedures.

  • Resources

    Software and Firmware

    Posters