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pFC17K HaloTag CMVd3 Flexi Vector

by Promega
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  • Overview
  • Protocols
  • Specifications
  • Resources
  • Constitutive Protein Expression in Mammalian Cells

    The pFC17A and pFC17K HaloTag® CMVd3 Flexi® Vectors are configured to append HaloTag® to the carboxyterminus of the protein fusion partner and provide constitutive protein expression in mammalian cells using a modified human cytomegalovirus (CMV) immediate-early enhancer/promoter, CMVd3. The vectors can be used for both stable and transient gene expression; for stable expression, cotransfection with a vector containing a selectable marker is required. A choice of ampicillin (A) or kanamycin (K) is provided with these vectors.

    Note: Flexi® Vectors contain the lethal barnase gene to reduce background colonies without inserts during the subcloning procedure. Using the Flexi® Vector Cloning System replaces the barnase gene with your insert. These vectors, as purchased, cannot be cultured in normal laboratory strains of E. coli without an insert. 

    Application

    • Clone ORF and conveniently transfer these inserts to a variety of vectors designed for multiple applications.

    GenBank® Accession Numbers and Sequence Information

    pFC17A HaloTag® CMVd3 GenBank® Accession Number EU332341; pFC17A HaloTag® CMVd3  Vector sequence text file.
    pFC17K HaloTag® CMVd3  GenBank® Accession Number EU332342; pFC17K HaloTag® CMVd3 Vector sequence text file.

  • Protocols

    Complete Protocol

    Download PDF

    pFC17A HaloTag® CMVd3 Flexi® Vector Protocol

    PDF (194 KB)

    Download PDF

    pFC17K HaloTag® CMVd3 Flexi® Vector Protocol

    PDF (197 KB)

    Video Protocols

    28440899-HALOTAG-ANIMATION-fallback
  • Certificate of Analysis

    Lookup Certificate of Analysis

    Storage Conditions

    -30C TO -10C

    Use Restrictions

    For Research Use Only. Not for Use in Diagnostic Procedures.

  • Resources

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