pFC17K HaloTag CMVd3 Flexi Vector

Constitutive Protein Expression in Mammalian Cells

The pFC17A and pFC17K HaloTag® CMVd3 Flexi® Vectors are configured to append HaloTag® to the carboxyterminus of the protein fusion partner and provide constitutive protein expression in mammalian cells using a modified human cytomegalovirus (CMV) immediate-early enhancer/promoter, CMVd3. The vectors can be used for both stable and transient gene expression; for stable expression, cotransfection with a vector containing a selectable marker is required. A choice of ampicillin (A) or kanamycin (K) is provided with these vectors.

Note: Flexi® Vectors contain the lethal barnase gene to reduce background colonies without inserts during the subcloning procedure. Using the Flexi® Vector Cloning System replaces the barnase gene with your insert. These vectors, as purchased, cannot be cultured in normal laboratory strains of E. coli without an insert. 

• Clone ORF and conveniently transfer these inserts to a variety of vectors designed for multiple applications.

GenBank® Accession Numbers and Sequence Information

pFC17A HaloTag® CMVd3 GenBank® Accession Number EU332341; pFC17A HaloTag® CMVd3  Vector sequence text file.
pFC17K HaloTag® CMVd3  GenBank® Accession Number EU332342; pFC17K HaloTag® CMVd3 Vector sequence text file.