pFC20K HaloTag T7 SP6 Flexi Vector
The pFN19 and pFC20 HaloTag® T7 SP6 Flexi® Vectors feature inducible expression of HaloTag® fusion proteins in E. coli and T7 and SP6 cell-free systems. Expression levels depend highly on the nature of the protein, but in general the N-terminal HaloTag® fusion protein can increase expression level, enhance refolding and boost solubility of the expressed protein.
pFN19A/K HaloTag® T7 SP6 Flexi® Vectors (Cat.# G1891, G1841) are designed for protein expression with an N-terminal HaloTag® using the T7 or SP6 RNA polymerase promoter. pFC20A/K HaloTag® T7 SP6 Flexi® Vectors (Cat.# G1681, G1691) express protein with a C-terminal HaloTag® using the T7 and SP6 RNA polymerase promoter. Proteins expressed from these vectors can be purified using HaloTag® technology.
About the Flexi® Vector System
The Flexi® Vector System is a simple, yet powerful, directional cloning method for protein-coding sequences. It is based on two rare-cutting restriction enzymes, SgfI and PmeI, and provides a rapid, efficient and high-fidelity way to transfer protein-coding regions between a variety of Flexi® Vectors without the need to resequence. Directly use recombinant clones and minimize time wasted screening background colonies. The versatile cloning of the Flexi® system means you can choose from a variety of expression systems and fusion tag orientations and then transfer to others as required. Direct transfers can only occur between two N-terminal tagged vectors or from an N-terminal to a C-terminal vector.
Note: Flexi® Vectors contain the lethal barnase gene to reduce background colonies without inserts during the subcloning procedure. Using the Flexi® Vector Cloning System replaces the barnase gene with your insert. These vectors, as purchased, cannot be cultured in normal laboratory strains of E. coli without an insert.
pFN19A HaloTag® T7 SP6 Flexi® Vector Protocol
PDF (198 KB)
pFN19K HaloTag® T7 SP6 Flexi® Vector Protocol
PDF (197 KB)
pFC20A HaloTag® T7 SP6 Flexi® Vector Protocol
PDF (199 KB)
pFC20K HaloTag® T7 SP6 Flexi® Vector Protocol
PDF (199 KB)
Certificate of AnalysisLookup Certificate of Analysis
-30C TO -10C
For Research Use Only. Not for Use in Diagnostic Procedures.
Software and Firmware