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pFC27A HaloTag CMV-neo Flexi Vector

by Promega
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  • Overview
  • Protocols
  • Specifications
  • Resources
  • Constitutive High-Level Protein Expression

    The pFC27A and pFC27K HaloTag® CMV-neo Flexi Vectors append HaloTag® protein to the carboxyterminus of the protein fusion partner. The vectors provide constitutive high-level protein expression in mammalian cells using the CMV immediate-early enhancer/promoter. The vectors can be used for both transient and stable gene expression. Stable expression is mediated by co-expression of the neomycin phosphotransferase gene, which confers resistance to the antibiotic G-418 Sulfate (Cat.# V7981) under the control of an SV40 promoter, allowing selection of stable transfectants. The vectors contain a choice of ampicillin (A) or kanamycin (K) antibiotic resistance.

    Note: Flexi® Vectors contain the lethal barnase gene to reduce background colonies without inserts during the subcloning procedure. Using the Flexi® Vector Cloning System replaces the barnase gene with your insert. These vectors, as purchased, cannot be cultured in normal laboratory strains of E. coli without an insert.

    • Clone ORF and conveniently transfer these inserts to a variety of vectors designed for multiple applications.

    GenBank® Accession Numbers and Sequence Information

    pFC27A HaloTag® CMV-neo Accession Number JN122283; pFC27A HaloTag® CMV-neo Vector sequence text file.
    pFC27K HaloTag® CMV-neo Accession Number JN122284; pFC27K HaloTag® CMV-neo Vector sequence text file.

  • Protocols

    Complete Protocol

    Download PDF

    pFC27A HaloTag CMV-neo Flexi Vector Product Information

    PDF (201 KB)

    Download PDF

    pFC27K HaloTag CMV-neo Flexi Vector Product Information

    PDF (202 KB)

    Video Protocols

  • Certificate of Analysis

    Lookup Certificate of Analysis

    Storage Conditions

    -30C TO -10C

    Use Restrictions

    For Research Use Only. Not for Use in Diagnostic Procedures.

  • Resources

    Software and Firmware