pFC27K HaloTag CMV-neo Flexi Vector

Constitutive High-Level Protein Expression

The pFC27A and pFC27K HaloTag® CMV-neo Flexi Vectors append HaloTag® protein to the carboxyterminus of the protein fusion partner. The vectors provide constitutive high-level protein expression in mammalian cells using the CMV immediate-early enhancer/promoter. The vectors can be used for both transient and stable gene expression. Stable expression is mediated by co-expression of the neomycin phosphotransferase gene, which confers resistance to the antibiotic G-418 Sulfate (Cat.# V7981) under the control of an SV40 promoter, allowing selection of stable transfectants. The vectors contain a choice of ampicillin (A) or kanamycin (K) antibiotic resistance.

Note: Flexi® Vectors contain the lethal barnase gene to reduce background colonies without inserts during the subcloning procedure. Using the Flexi® Vector Cloning System replaces the barnase gene with your insert. These vectors, as purchased, cannot be cultured in normal laboratory strains of E. coli without an insert.

• Clone ORF and conveniently transfer these inserts to a variety of vectors designed for multiple applications.

GenBank® Accession Numbers and Sequence Information

pFC27A HaloTag® CMV-neo Accession Number JN122283; pFC27A HaloTag® CMV-neo Vector sequence text file.
pFC27K HaloTag® CMV-neo Accession Number JN122284; pFC27K HaloTag® CMV-neo Vector sequence text file.