pFN21A HaloTag CMV Flexi Vector
Constitutive Protein Expression in Mammalian Cells
The pFN21A and pFN21K HaloTag® CMV Flexi® Vectors are configured to append HaloTag® protein tag to the aminoterminus of the protein fusion partner and provide constitutive protein expression in mammalian cells using the human cytomegalovirus (CMV) immediate-early enhancer/promoter. The vectors can be used for both stable and transient gene expression; for stable expression, cotransfection with a vector containing a selectable marker is required. The vectors are provided with a choice of ampicillin (A) or kanamycin (K) antibiotic resistance genes.
Note: Flexi® Vectors contain the lethal barnase gene to reduce background colonies without inserts during the subcloning procedure. Using the Flexi® Vector Cloning System replaces the barnase gene with your insert. These vectors, as purchased, cannot be cultured in normal laboratory strains of E. coli without an insert.Application
- Clone ORF and conveniently transfer these inserts to a variety of vectors designed for multiple applications.
GenBank® Accession Numbers and Sequence Information
pFN21A HaloTag® CMV GenBank® Accession Number EU621374; pFN21A HaloTag® CMV Vector sequence text file.
pFN21K HaloTag® CMV GenBank® Accession Number EU621375; pFN21K HaloTag® CMV Vector sequence text file.
pFN21A HaloTag® CMV Flexi® Vector Protocol
PDF (192 KB)
pFN21K HaloTag® CMV Flexi® Vector Protocol
PDF (192 KB)
Certificate of AnalysisLookup Certificate of Analysis
-30C TO -10C
For Research Use Only. Not for Use in Diagnostic Procedures.
Software and Firmware