pFN28A HaloTag CMV-neo Flexi Vector
Constitutive Protein Expression in Mammalian Cells
The pFN28A and pFN28K HaloTag® CMV-neo Flexi Vectors append the HaloTag® protein to the aminoterminus of the protein fusion partner. The vectors provide constitutive high-level protein expression in mammalian cells using the CMV immediate-early enhancer/promoter. The vectors can be used for both transient and stable gene expression. The stable expression is mediated by co-expression of the neomycin phosphotransferase gene, which confers resistance to the Antibiotic G-418 Sulfate (Cat.# V7981) under the control of an SV40 promoter, allowing selection of stable transfectants.
Note: Flexi® Vectors contain the lethal barnase gene to reduce background colonies without inserts during the subcloning procedure. Using the Flexi® Vector Cloning System replaces the barnase gene with your insert. These vectors, as purchased, cannot be cultured in normal laboratory strains of E. coli without an insert.Application
- Clone ORF and conveniently transfer these inserts to a variety of vectors designed for multiple applications.
GenBank® Accession Numbers and Sequence Information
pFN28A HaloTag® CMV-neo GenBank® Accession Number JN129497; pFN28A HaloTag® CMV-neo Vector sequence text file.
pFN28K HaloTag® CMV-neo GenBank® Accession Number JN129498; pFN28K HaloTag® CMV-neo Vector sequence text file.
pFN28A HaloTag CMV-neo Flexi Vector Product Information
PDF (204 KB)
pFN28K HaloTag® CMV-neo Flexi® Vector Product Information
PDF (200 KB)
Certificate of AnalysisLookup Certificate of Analysis
-30C TO -10C
For Research Use Only. Not for Use in Diagnostic Procedures.
Software and Firmware