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pFN2K (GST) Flexi Vector

by Promega
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  • Overview
  • Protocols
  • Specifications
  • Resources
  • The pFN2A and pFN2K (GST) Flexi® Vectors are used for inducible expression of GST-tagged fusion proteins in E. coli and cell-free systems using the T7 RNA polymerase promoter. The GST tag has been successfully used to boost tagged protein solubility during E. coli expression. Reusable gluthathione (GST-affinity) resins can be used with the pFN2 (GST) Flexi® Vectors for standard protein purification and prokaryotic expression applications. Express cloned GST-tagged ORFs in bacterial and cell-free systems.

    About the Flexi® Vector System

    The Flexi® Vector System is a simple, yet powerful, directional cloning method for protein-coding sequences. It is based on two rare-cutting restriction enzymes, SgfI and PmeI, and provides a rapid, efficient and high-fidelity way to transfer protein-coding regions between a variety of Flexi® Vectors without the need to resequence. Directly use recombinant clones and minimize time wasted screening background colonies. The versatile cloning of the Flexi® system means you can choose from a variety of expression systems and fusion tag orientations and then transfer to others as required. Direct transfers can only occur between two N-terminal tagged vectors or from an N-terminal to a C-terminal vector.

    Note: Flexi® Vectors contain the lethal barnase gene to reduce background colonies without inserts during the subcloning procedure. Using the Flexi® Vector Cloning System replaces the barnase gene with your insert. These vectors, as purchased, cannot be cultured in normal laboratory strains of E. coli without an insert.

    Sequence Information

    pFN2A (GST) Flexi® Vector GenBank® Accession Number AY753578 and vector sequence text file.

    pFN2K (GST) Flexi® Vector GenBank® Accession Number AY753579 and vector sequence text file.

  • Protocols

    Complete Protocol

    Download PDF

    pFN2A (GST) Flexi® Vector Product Information

    PDF (134 KB)

    Download PDF

    pFN2K (GST) Flexi® Vector Product Information

    PDF (132 KB)

    Video Protocols

  • Certificate of Analysis

    Lookup Certificate of Analysis

    Storage Conditions

    -30C TO -10C

    Use Restrictions

    For Research Use Only. Not for Use in Diagnostic Procedures.

  • Resources


    Software and Firmware