pFN39K secHiBiT CMV-neo Flexi Vector
The three HiBiT CMV-neo Flexi® Vectors are configured to facilitate simple, efficient transfer of the gene of interest into a vector designed for genetic attachment of the HiBiT peptide tag to the amino or carboxy terminus of the protein of interest using the Flexi® Cloning System (Cat.# C8640). The vectors can be used for both stable and transient gene expression and encode kanamycin resistance for bacterial selection and neomycin resistance for mammalian selection.
The pFC37K HiBiT CMV-neo Flexi® Vector appends the HiBiT to the C terminus of the gene of interest.
The pFN38K HiBiT CMV-neo Flexi® Vector adds the HiBiT tag to the N terminus of the gene of interest.
The pFN39K secHiBiT CMV-neo Flexi® Vector attaches the HiBiT tag to the N terminus of the gene of interest. This vector encodes the IL-6 secretion signal peptide N-terminal to the HiBiT tag for direct trafficking of HiBiT-tagged proteins to the plasma membrane of mammalian cells for cell surface expression and secreted proteins.
Note: We recommend removing naturally encoded secretion signal sequences from the gene of interest for efficient cell-surface expression of the HiBiT-tagged protein.
The HiBiT peptide tag, in combination with the Nano-Glo® HiBiT detection systems, offers bioluminescent detection of the protein of interest. This results in quantitation of proteins using bioluminescence with no need for antibodies.
- Expression of the HiBiT-tagged protein will only occur when the proper reading frame is maintained between the HiBiT tag and the gene of interest.
- The HiBiT peptide sequence is provided for reference only. To obtain rights to synthesize the HiBiT tag, please see the Terms and Conditions of Use.
About the Flexi® Vector System
The Flexi® Vector System is a simple, yet powerful, directional cloning method for protein-coding sequences. It is based on two rare-cutting restriction enzymes, SgfI and PmeI, and provides a rapid, efficient and high-fidelity way to transfer protein-coding regions between a variety of Flexi® Vectors without the need to resequence. Directly use recombinant clones and minimize time wasted screening background colonies. The versatile cloning of the Flexi® system means you can choose from a variety of expression systems and fusion tag orientations and then transfer to others as required. Direct transfers can only occur between two N-terminal tagged vectors or from an N-terminal to a C-terminal vector.
Note: Flexi® Vectors contain the lethal barnase gene to reduce background colonies without inserts during the subcloning procedure. Using the Flexi® Vector Cloning System replaces the barnase gene with your insert. These vectors, as purchased, cannot be cultured in normal laboratory strains of E. coli without an insert.
Ready-to-Use HaloTag® Flexi® Vector Clones
For access to 20,000 human and 15,000 mouse ORF clones with HaloTag® Technology in multiple mammalian expression vectors and custom cloning options, visit: www.kasuza.or.jp.kop/dsearch-e
pFC37K HiBiT CMV-neo Flexi® Vector Product Information
PDF (156 KB)
pFN38K HiBiT CMV-neo Flexi® Vector Product Information
PDF (156 KB)
pFN39K secHiBiT CMV-neo Flexi® Vector Product Information
PDF (156 KB)
Certificate of AnalysisLookup Certificate of Analysis
-30C TO -10C
For Research Use Only. Not for Use in Diagnostic Procedures.