- Overview
- Protocols
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The pGEM®-4Z Vector is a standard cloning vector that can also efficiently synthesize RNA in vitro. The vector carries the lacZ ?-peptide and the multiple cloning region arrangement from pUC18 for selecting recombinants by blue/white screening. In addition, the vector contains both the SP6 and T7 RNA polymerase promoters flanking the multiple cloning region.
The pGEM®-3Z and pGEM®-4Z Vectors are essentially identical except for the orientation of the SP6 and T7 promoters.
Reference
- Yanisch-Perron, C. et al. (1985) Gene 33, 103–19.
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Protocols
Complete Protocol
pGEM®-4Z Vector Technical Bulletin
PDF (275 KB)
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Certificate of Analysis
Lookup Certificate of AnalysisStorage Conditions
LESS THAN -65C
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.
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Resources
Citations
