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pGEM-9Zf(-) Vector

by Promega
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  • Overview
  • Protocols
  • Specifications
  • Resources
  • The pGEM®-9Zf(–) Vector is a recombinant plasmid designed to provide a versatile range of cloning strategies, and contains T7 and SP6 RNA polymerase promoters flanking a multiple cloning region within the ?-peptide coding region of ?-galactosidase. Insertional inactivation of the ?-peptide identifies recombinant clones by color screening on indicator plates when using appropriate E. coli strains (e.g., JM109). The multiple cloning region is unique and includes restriction sites for NsiI, SpeI, HindIII, XbaI, EcoRI, SalI and SacI.

    0288VA05_4A-W

    pGEM®-9Zf(–) Vector GenBank® Accession Number X65312 and vector sequence text file.

  • Protocols

    Complete Protocol

    Download PDF

    pGEM®-9Zf(–) Vector Technical Bulletin

    PDF (349 KB)

  • Certificate of Analysis

    Lookup Certificate of Analysis

    Storage Conditions

    LESS THAN -65C

    Use Restrictions

    For Research Use Only. Not for Use in Diagnostic Procedures.

  • Resources

    Citations