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pGL2-Promoter Vector

by Promega
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  • Overview
  • Protocols
  • Specifications
  • Resources
  • The pGL2 Luciferase Reporter Vectors provide a basis for the quantitative analysis of factors that potentially regulate mammalian gene expression. These factors may be cis-acting, such as promoters and enhancers, or trans-acting, such as various DNA-binding factors. The pGL2 Vectors carry the coding region for firefly (Photinus pyralis) luciferase, which is used to monitor transcriptional activity in transfected eukaryotic cells. The assay of this genetic reporter is rapid, sensitive and quantitative. In addition, the pGL2 Vectors contain numerous features that aid in the characterization and mutagenesis of the putative regulatory sequences.

    About the Vector Options

    The reporter vectors are available in four options, depending on your needs. The Basic Vector has the reporter gene and restriction enzymes sites to clone in a promoter of interest. The Enhancer Vector contains the SV40 Enhancer Region for putative promoters that may need an additional regulator region. The Promoter Vector is suitable for cloning and testing putative enhancer regions. The Control Vector contains the SV40 Promoter and Enhancer Region and can be used as a normalization control for expression experiments.


    pGL2-Basic Vector GenBank® Accession Number X65323 and vector sequence text file.


    pGL2-Control Vector GenBank® Accession Number X65324 and vector sequence text file.


    pGL2-Enhancer Vector GenBank® Accession Number X65325  and vector sequence text file.


    pGL2-Promoter Vector GenBank® Accession Number X65326 and vector sequence text file.

  • Protocols

    Complete Protocol

    Download PDF

    pGL2 Luciferase Reporter Vectors Technical Manual

    PDF (1 MB)

  • Certificate of Analysis

    Lookup Certificate of Analysis

    Storage Conditions

    LESS THAN -65C

    Use Restrictions

    For Research Use Only. Not for Use in Diagnostic Procedures.

  • Resources