10X Flexi Enzyme Blend (SgfI & PmeI)

by Promega
Available SKUs: R1851 | R1852 |
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Description

  • Overview
  • Protocols
  • Specifications
  • Resources
  • The 10X Flexi® Enzyme Blend (SgfI and PmeI) is required to screen Flexi® Vector clones for the presence of the protein-coding region of interest. This enzyme blend can also be used to cleave PCR products prior to cloning into the Flexi® Vectors or transfer protein-coding regions between Flexi® Vectors. For more information see the Flexi® Vector Systems Technical Manual #TM254.

    Recognition Sites:
    SgfI recognition site:
    5´…GCGATtCGC…3´
    3´…CGCsTAGCG…5´

    PmeI recognition site:
    5´…GTTTtAAAC…3´
    3´…CAAAsTTTG…5´

    Storage Buffer: The 10X Flexi® Enzyme Blend (SgfI and PmeI) is supplied in 10mM Tris-HCl (pH 7.4 at 25°C), 150mM KCl, 50mM NaCl, 0.1mM EDTA, 1mM DTT, 0.35mg/ml BSA, 0.05% Thesit® and 50% glycerol.

    5X Flexi® Digest Buffer: The composition of 5X Flexi® Digest Buffer is 50mM Tris-HCl (pH 7.9 at 37°C), 50mM MgCl2, 250mM NaCl, 5mM DTT, 0.5mg/ml acetylated BSA.

  • Certificate of Analysis

    Lookup Certificate of Analysis

    Storage Conditions

    -30C TO -10C

    Use Restrictions

    For Research Use Only. Not for Use in Diagnostic Procedures.

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