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pGL4.14[luc2/Hygro] Vector

by Promega
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  • Overview
  • Protocols
  • Specifications
  • Resources
  • The pGL4.14[luc2/Hygro], pGL4.15[luc2P/Hygro] and pGL4.16[luc2CP/Hygro] Vectors are designed primarily to accept a putative promoter element for investigation of important regions controlling gene transcription. The promoterless vectors are available with three varieties of engineered firefly luciferase genes: luc2, luc2P or luc2CP. These pGL4 vectors have the selectable marker, hygromycin. The inserts cloned into these vectors can easily be transferred using the multiple cloning site and a unique SfiI transfer scheme.

    Applications

    • Transcription regulation.
    • Virus-cell interactions.
    • Compound screening.
    • Post-translational modifications.
    • Promoter analysis.

    How the Engineered Firefly Luciferase Genes Work

    The luc2 gene is engineered to remove most cryptic transcription factor binding sites and improve mammalian expression through codon optimization. The luc2P and luc2CP and RapidResponse™ genes are luc2 genes appended with degradation sequences to influence the cellular half-life of firefly luciferase. The RapidResponse™ genes respond more rapidly to stimuli but at the expense of signal intensity. The luc2P (1-hour half-life) gene responds more rapidly than luc2 (3-hour half-life) with moderate signal intensity, and the luc2CP (0.4-hour half-life) responds more quickly with the lowest signal intensity.

    About the pGL4 Reporter Vectors

    The pGL4 Vectors offer increased reporter gene expression with codon optimization of synthetic genes for mammalian expression and reduced background and risk of expression artifacts with removal of cryptic DNA regulatory elements and transcription factor binding sites. Firefly luciferase has options that offer improved temporal response with the destabilized Rapid Response™ luciferase genes.

    4862MA-W

    pGL4.14[luc2/Hygro] Vector GenBank® Accession Number AY864928 and vector sequence text file.

    4863MA-W

    pGL4.15[luc2P/Hygro] Vector GenBank® Accession Number AY864929 and vector sequence text file.

    4864MA-W

    pGL4.16[luc2CP/Hygro] Vector GenBank® Accession Number AY864930 and vector sequence text file.

  • Protocols

    Complete Protocol

    Download PDF

    pGL4.14[luc2/Hygro] Vector Protocol

    PDF (186 KB)

    Download PDF

    pGL4.15[luc2P/Hygro] Vector Protocol

    PDF (144 KB)

    Download PDF

    pGL4.16[luc2CP/Hygro] Vector Protocol

    PDF (185 KB)

  • Certificate of Analysis

    Lookup Certificate of Analysis

    Storage Conditions

    -30C TO -10C

    Use Restrictions

    For Research Use Only. Not for Use in Diagnostic Procedures.

  • Resources

    Citations