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pGL4.25[luc2CP/minP] Vector

by Promega
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  • Overview
  • Protocols
  • Specifications
  • Resources
  • Minimal Promoter-Driven Firefly Luciferase Vectors

    Creating a cell line with an indicator of a functional signaling pathway is useful for deciphering the components in a signaling pathway. These tools are made by insertion of multiple repeats of a response element upstream of a minimal promoter (minP).

    To construct pathway reporters, the pGL4.23[luc2/minP], pGL4.24[luc2P/minP] and pGL4.25[luc2CP/minP] Vectors have minimal promoter (minP) vectors with three varieties of engineered firefly luciferase genes. The luc2 gene is engineered to remove most cryptic transcription factor binding sites and improve mammalian expression through codon optimization. The luc2P and luc2CP and RapidResponse™ genes are luc2 genes appended with degradation sequences to influence the cellular half-life of firefly luciferase. Proteins encoded by the RapidResponse™ genes respond more rapidly to stimuli but at the expense of signal intensity. The luc2P (1-hour half-life) gene responds more rapidly than luc2 (3-hour half-life) with moderate signal intensity, and the luc2CP (0.4-hour half-life) responds more quickly with the lowest signal intensity.

    We have designed vectors that report the activity of a variety of pathways using the optimized firefly luciferase gene luc2 in the pGL4 backbone. Learn about the available vectors and the pathways each can measure. Some of these pre-designed response element vectors also are available stable cell lines (GloResponse™ Cell Lines) to speed your research.

    Applications

    • Transcription regulation.
    • Virus-cell interactions.
    • Compound screening.
    • Post-translational modifications.
    • GPCR signaling.
    • Cell signaling.

    About the pGL4 Reporter Vectors

    The pGL4 Vectors offer increased reporter gene expression with codon optimization of synthetic genes for mammalian expression and reduced background and risk of expression artifacts with removal of cryptic DNA regulatory elements and transcription factor binding sites. Firefly luciferase has options that offer improved temporal response with the destabilized Rapid Response™ luciferase genes.

    5948MA-W

    pGL4.23[luc2/minP] Vector GenBank® Accession Number DQ904455 and vector sequence text file.

    5949MA-W

    pGL4.24[luc2P/minP] Vector GenBank® Accession Number DQ904456 and vector sequence text file.

    5950MA-W

    pGL4.25[luc2CP/minP] Vector GenBank® Accession Number DQ904457 and vector sequence text file.

  • Protocols

    Complete Protocol

    Download PDF

    pGL4.23[luc2/minP] Vector Protocol

    PDF (184 KB)

    Download PDF

    pGL4.24[luc2P/minP] Vector Protocol

    PDF (172 KB)

    Download PDF

    pGL4.25[luc2CP/minP] Vector Protocol

    PDF (173 KB)

  • Certificate of Analysis

    Lookup Certificate of Analysis

    Storage Conditions

    -30C TO -10C

    Use Restrictions

    For Research Use Only. Not for Use in Diagnostic Procedures.

  • Resources

    Citations