Creating a cell line with an indicator of a functional signaling pathway is useful for deciphering the components in a signaling pathway. These tools are made by insertion of multiple repeats of a response element upstream of a minimal promoter (minP). Promega has designed vectors that report the activity of a variety of pathways using the optimized luc2 firefly luciferase gene in the pGL4 backbone. These vectors also have a hygromycin resistance selectable marker, allowing use either in transient transfection experiments or for selection of a stable cell line. In addition, Some of these pre-designed response element vectors also are available stable cell lines (GloResponse™ Cell Lines) to speed your research.
- Cell transcription.
- Virus-cell interactions.
- Compound screening.
- Post-translational modifications.
- GPCR signaling.
- Cell signaling.
About the pGL4 Reporter Vectors
The pGL4 Vectors offer increased reporter gene expression with codon optimization of synthetic genes for mammalian expression and reduced background and risk of expression artifacts with removal of cryptic DNA regulatory elements and transcription factor binding sites. Firefly luciferase has options that offer improved temporal response with the destabilized Rapid Response™ luciferase genes.
Activator/Pathway Transcription Factor Binding Site Vector Name Example Data Oxidative stress Nrf2 Antioxidant Response Element (ARE) pGL4.37[luc2P/ARE/Hygro] DNA damage
p53 response element (p53 RE) pGL4.38[luc2P/p53 RE/Hygro] Endoplasmic reticulum stress ATF6 Activating Transcription Factor 6 response element (ATF6 ERSE) pGL4.39[luc2P/ATF6 RE/Hygro] Heavy metal stress MTF1 Metal Regulatory Element (MRE) pGL4.40[luc2P/MRE/Hygro] Heat shock HSF1 Heat Shock Element (HSE) pGL4.41[luc2P/HSE/Hygro] Hypoxia Hif1? Hypoxia Response Element (HRE) pGL4.42[luc2P/HRE/Hygro] Xenobiotic stress
Xenobiotic Responsive Element (XRE) pGL4.43[luc2P/XRE/Hygro]
pGL4.37[luc2P/ARE/Hygro] Vector Protocol
PDF (198 KB)
pGL4.38[luc2P/p53 RE/Hygro] Vector Protocol
PDF (206 KB)
pGL4.39[luc2P/ATF6 RE/Hygro] Vector Protocol
PDF (192 KB)
pGL4.40[luc2P/MRE/Hygro] Vector Protocol
PDF (212 KB)
pGL4.41[luc2P/HSE/Hygro] Vector Protocol
PDF (197 KB)
pGL4.42[luc2P/HRE/Hygro] Vector Protocol
PDF (194 KB)
pGL4.43[luc2P/XRE/Hygro] Vector Protocol
PDF (215 KB)
Certificate of AnalysisLookup Certificate of Analysis
-30C TO -10C
For Research Use Only. Not for Use in Diagnostic Procedures.