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pGL4.51[luc2/CMV/Neo] Vector

by Promega
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  • Overview
  • Protocols
  • Specifications
  • Resources
  • The pGL4.50[luc2/CMV/Hygro] and pGL4.51[luc2/CMV/Neo] Vectors are part of the pGL4 next generation reporter gene vectors optimized for expression in mammalian cells. Once these vectors are transfected into cultured cells, the luciferase-expressing cells can be used in animal imaging studies. The pGL4.50 and pGL4.51 Vectors have the synthetic firefly luciferase luc2 gene under the control of the strong constitutive CMV (cytomegalovirus) promoter. These vectors have demonstrated high expression levels in a variety of cell lines tested. The selectable marker options, either hygromycin or neomycin, can generate stable cell lines. Cell lines with constant expression of luciferase can be used in animal models to study in vivo changes in cell physiology.

    Interested in other pGL4 Reporter Vectors? Browse them on the Reporter Vectors and Cell Lines web page.


    pGL4.50[luc2/CMV/Hygro] Vector GenBank® Accession Number EU921840 and vector sequence text file.


    pGL4.51[luc2/CMV/Neo] Vector GenBank® Accession Number  EU921841 and vector sequence text file.

  • Protocols

    Complete Protocol

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    pGL4.50[luc2/CMV/Hygro] Vector

    PDF (159 KB)

    Download PDF

    pGL4.51[luc2/CMV/Neo] Vector Protocol

    PDF (160 KB)

  • Certificate of Analysis

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    Storage Conditions

    -30C TO -10C

    Use Restrictions

    For Research Use Only. Not for Use in Diagnostic Procedures.

  • Resources