pGL4.53[luc2/PGK] Vector

The promoter-driven firefly control vectors are commonly co-transfected with experimental NanoLuc® or  Renilla luciferase vectors for use in dual-reporter assays such as the Dual-Luciferase® or Dual-Glo® Reporter Assay Systems. The control vectors should give an almost invariant level of activity, while the experimental Renilla vector varies with treatment. The luc2 gene that encodes firefly luciferase is optimized for mammalian expression. If the CMV promoter is desired, the pGL4.50[luc2/CMV/Hygro] and pGL4.51[luc2/CMV/Neo] Vectors are useful for tagging a cell line and offer a selectable marker for creating stable transfectants.

Applications

• Transcription regulation.
• Virus-cell interactions.
• Compound screening.
• Post-translational modifications.
• GPCR signaling.
• Cell signaling.
• Promoter analysis.

About the pGL4 Reporter Vectors

The pGL4 Vectors offer increased reporter gene expression with codon optimization of synthetic genes for mammalian expression and reduced background and risk of expression artifacts with removal of cryptic DNA regulatory elements and transcription factor binding sites. Firefly luciferase has options that offer improved temporal response with the destabilized Rapid Response™ luciferase genes.

Sequence Information

pGL4.13[luc2/SV40] Vector GenBank® Accession Number AY738225 and vector sequence text file.

pGL4.53[luc2/PGK] Vector GenBank® Accession Number KM359768 and vector sequence text file.

pGL4.54[luc2/TK] Vector GenBank® Accession Number KM359769 and vector sequence text file.