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pH6HTC His6HaloTag T7 Vector

by Promega
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  • Overview
  • Protocols
  • Specifications
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  • The pH6HTN and pH6HTC His6HaloTag® T7 Vectors are used for inducible expression of polyhistidine-HaloTag® fusion proteins in E. coli and cell-free systems using the T7 RNA polymerase promoter. Expression levels depend highly on the nature of the protein, but in general the N-terminal HaloTag® fusion protein can increase expression level, enhance refolding and boost solubility of the expressed protein. HaloTag® vectors are supplied as multiple cloning site (MCS) vectors for traditional cloning.

    pH6HTN His6HaloTag® T7 Vector (Cat.# G7971) is designed for protein expression with an N-terminal His6-HaloTag® dual tag; pH6HTC His6HaloTag® T7 Vector (Cat.# G8031) is designed for protein expression with a C-terminal His6-HaloTag® dual tag. By coupling the protein solubility and labeling benefits of HaloTag® technology with the reusability and the throughput of Ni-affinity technology, the dual tag enables protein purification with the reusable Ni-resin while retaining the HaloTag® covalent binding with HaloTag® ligands.

    10317MA-W

    pH6HTC His6HaloTag® T7 Vector GenBank® Accession Number JN874647 and vector sequence text file.

    10318MA-W

    pH6HTN His6HaloTag® T7 Vector GenBank® Accession Number JN874646 and vector sequence text file.

    HaloTag® Technology improves purification of difficult proteins.
    HaloTag® Technology improves purification of difficult proteins.
  • Protocols

    Complete Protocol

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    pH6HTC His6HaloTag® T7 Vector Product Information

    PDF (215 KB)

    Download PDF

    pH6HTN His6HaloTag® T7 Vector Product Information

    PDF (211 KB)

  • Certificate of Analysis

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    Storage Conditions

    -30C TO -10C

    Use Restrictions

    For Research Use Only. Not for Use in Diagnostic Procedures.

  • Resources

    Software and Firmware