pH6HTC His6HaloTag T7 Vector
The pH6HTN and pH6HTC His6HaloTag® T7 Vectors are used for inducible expression of polyhistidine-HaloTag® fusion proteins in E. coli and cell-free systems using the T7 RNA polymerase promoter. Expression levels depend highly on the nature of the protein, but in general the N-terminal HaloTag® fusion protein can increase expression level, enhance refolding and boost solubility of the expressed protein. HaloTag® vectors are supplied as multiple cloning site (MCS) vectors for traditional cloning.
pH6HTN His6HaloTag® T7 Vector (Cat.# G7971) is designed for protein expression with an N-terminal His6-HaloTag® dual tag; pH6HTC His6HaloTag® T7 Vector (Cat.# G8031) is designed for protein expression with a C-terminal His6-HaloTag® dual tag. By coupling the protein solubility and labeling benefits of HaloTag® technology with the reusability and the throughput of Ni-affinity technology, the dual tag enables protein purification with the reusable Ni-resin while retaining the HaloTag® covalent binding with HaloTag® ligands.
pH6HTC His6HaloTag® T7 Vector Product Information
PDF (215 KB)
pH6HTN His6HaloTag® T7 Vector Product Information
PDF (211 KB)
Certificate of AnalysisLookup Certificate of Analysis
-30C TO -10C
For Research Use Only. Not for Use in Diagnostic Procedures.
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