pH6HTC His6HaloTag T7 Vector

The pH6HTN and pH6HTC His₆HaloTag® T7 Vectors are used for inducible expression of polyhistidine-HaloTag® fusion proteins in E. coli and cell-free systems using the T7 RNA polymerase promoter. Expression levels depend highly on the nature of the protein, but in general the N-terminal HaloTag® fusion protein can increase expression level, enhance refolding and boost solubility of the expressed protein. HaloTag® vectors are supplied as multiple cloning site (MCS) vectors for traditional cloning.

pH6HTN His₆HaloTag® T7 Vector (Cat.# G7971) is designed for protein expression with an N-terminal His₆-HaloTag® dual tag; pH6HTC His₆HaloTag® T7 Vector (Cat.# G8031) is designed for protein expression with a C-terminal His₆-HaloTag® dual tag. By coupling the protein solubility and labeling benefits of HaloTag® technology with the reusability and the throughput of Ni-affinity technology, the dual tag enables protein purification with the reusable Ni-resin while retaining the HaloTag® covalent binding with HaloTag® ligands.

pH6HTC His₆HaloTag® T7 Vector GenBank® Accession Number JN874647 and vector sequence text file.

pH6HTN His₆HaloTag® T7 Vector GenBank® Accession Number JN874646 and vector sequence text file.