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PhiX174, RF DNA

by Promega
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  • Overview
  • Protocols
  • Specifications
  • Resources
  • The icosahedral bacteriophage ?X174 replicative form (RF) is a double-stranded circular DNA molecule of 5,386 bases. Restriction enzyme-digested ?X174 DNA generates molecular weight size markers used in gel analysis of nucleic acids. ?X174 DNA is often used in the assays of restriction enzymes for the presence of nickase activity.

    Storage Buffer: 10mM Tris-HCl (pH 7.5), 1mM EDTA.

    GenBank - EMBL Accession Number: NC001422.


    1. Sanger, F. et al. (1978) J. Mol. Biol. 125, 225–46.
  • Certificate of Analysis

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    Storage Conditions

    -30C TO -10C

    Use Restrictions

    For Research Use Only. Not for Use in Diagnostic Procedures.